bac.water (Sterile Water for Injection, SWFI)

Important Clarification: Sterile Water is NOT a peptide. It is a preservative-free aqueous solvent widely used to reconstitute lyophilized research peptides.

1. Basic Overview

• Full Name: Sterile Water for Injection (SWFI)
• Chemical Composition: Purified, sterile, pyrogen-free H₂O; no preservatives, no benzyl alcohol
• Appearance: Clear, colorless liquid
• Common Pack Specification: 5mL, 10mL, 30mL glass vials
• Core Feature: Zero additives. Low cytotoxicity, compatible with in vitro cell culture and short-term in vivo animal experiments.

2. Mechanism & Characteristics

1. Inert aqueous solvent

   Neutral pH range, dissolves most lyophilized peptides without altering peptide molecular structure.

2. Preservative-Free

   No bacteriostatic agents. Cannot suppress microbial growth once the rubber stopper is punctured.

3. Biocompatible

   Unlike Bacteriostatic Water (BAC Water) containing benzyl alcohol, sterile water does not cause cell toxicity, making it suitable for cell culture experiments.

3. Main Research Applications

• Reconstitution of lyophilized peptides for in vitro cell culture testing
• Single-dose short-term animal injection experiments
• Experiments sensitive to benzyl alcohol interference
• Dilution buffer preparation for biochemical assays

Limitation: Not suitable for multi-dose long-term storage. Risk of bacterial contamination after opening.

4. Reconstitution Operation Protocol

1. Let lyophilized peptide vial reach room temperature. Disinfect stoppers of both vials with alcohol pads.
2. Draw required volume of sterile water with a syringe.
3. Slowly inject liquid along the inner wall of peptide vial. Avoid direct impact onto powder to prevent peptide denaturation and foaming.
4. Gently swirl the vial to dissolve powder. Do not shake vigorously.

5. Storage Rules

• Unopened vial: Store at 15–30°C, avoid direct strong light, follow manufacturer expiration date.
• After rubber stopper pierced: Use within 24–72 hours; discard leftover liquid.
• Reconstituted peptide solution: Store at 2–8°C; freezing is not recommended.

6. Precautions

1. Laboratory experimental solvent only. Not for human medical use.
2. Once opened, bacteria will reproduce rapidly. Never store reconstituted peptides for up to 28 days like BAC Water.
3. Discard liquid if turbidity, suspended particles or discoloration occurs.
4. Not suitable for peptides requiring long-term stability testing without sterile sealing.
5. Strict aseptic operation is required during all transfer procedures.

7. Comparison: Sterile Water VS Bacteriostatic Water (BAC Water)

Short Version (For Product Listing / Customer Message)

Sterile Water (Sterile Water for Injection)